Postdoctoral Research Fellow

3 days ago

Jeddah, Saudi Arabia KAUST Full time

Postdoctoral Research Fellow - Genome Engineering with CRISPR/Cas systems- King Abdullah University of Science and Technology: Postdoc Positions: Biological and Environmental Science and Engineering Division (postdoc): Bioscience and Bioengineering**Location**:

- Thuwal, Saudi Arabia**Open Date**:

- Jun 07, 2018**Description**:
The Mahfouz laboratory for genome engineering at KAUST has two postdoctoral positions in the area crop bioengineering via CRISPR/Cas systems. The goal of our research is to develop innovative tools for efficient genome engineering for targeted improvement of crop traits.

**These postdoctoral research positions are focused on the following research projects**:

- Tissue culture-free genome engineering via CRISPR/Cas9 system.
- Foreign DNA-free cereal genome engineering.
- Targeted insertions and gene stacking via HDR in cereal genomes.

These positions are available immediately and remain open until filled.

**Qualifications**:
**Required qualifications**:

- A Ph.D. in molecular plant science, biochemistry, biotechnology, molecular biology or related field is required.
- Demonstrated strong record of research productivity as evidenced by good quality publications.
- Effective writing and presentation skills.

**You are expected to have expertise to efficiently perform the following duties**:

- Design, build and engineer a variety of plasmid constructs including binary vectors.
- Transient transformation into tobacco plants via agroinfection to test the designs and catalytic activity of the CRISPR/Cas machinery.
- Generate stable expression lines with CRISPR/Cas9 machinery in model and non-model plant species.
- Use of Agrobacterium and gene gun for plant transformation.
- Establish germline engineering in crop species.
- Genotyping of edited plants using T7EI, Sanger sequencing and restriction enzyme loss mutation detection assays.
- Genome wide gene editing via sgRNA libraries.
- Analysis of deep amplicon sequencing data.
- Phenotypic assays of edited plant species.
- Present and publish research findings.

**Application Instructions**:
Applications should include:

- A full CV with publication list,
- A 2-5-page statement of research interests
- Contact information for three academic references.

**Application Process**:

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